SrasV1, Main, Exploration, bibRecord, 004E57

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods

Identifieur interne : 004E57 ( Main/Exploration ); précédent : 004E56; suivant : 004E58

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods

Auteurs : W. C. Yam [Hong Kong] ; K. H. Chan [Hong Kong] ; K. H. Chow [Hong Kong] ; L. L. M. Poon [Hong Kong] ; H. Y. Lam [Hong Kong] ; K. Y. Yuen [Hong Kong] ; W. H. Seto [Hong Kong] ; J. S. M. Peiris [Hong Kong]

Source :

Journal of clinical virology [ 1386-6532 ] ; 2005.

RBID : Pascal:05-0205858

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Temps réel, Réaction chaîne polymérase, Diagnostic, Epidémie, Microbiologie, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Diagnosis, Epidemic, Microbiology, Polymerase chain reaction, Real time, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Virology.

Abstract

Background: The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease. Objective: To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong. Study design: Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV. Results: In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n=44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens. Conclusion: This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.

Affiliations:

Hong Kong

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Background: The protocols of WHO network laboratories facilitated development of rapid diagnosis for SARS coronavirus (CoV) using reverse transcription (RT)-PCR assays. However, several reports have shown that conventional and real-time PCR assays were very specific for SARS CoV but lack sensitivity depending on the assay, specimen, and time course of disease. Objective: To evaluate an automatic nucleic acid extraction system and two standardized real-time PCR assays for rapid diagnosis of SARS CoV during outbreak and post-epidemic periods in Hong Kong. Study design: Specimens from clinically suspected SARS patients collected during outbreak and post-epidemic periods were tested by an automatic nucleic acid extraction system followed by our first generation conventional RT-PCR and two standardized real-time PCR assays (Artus GmbH, Germany and Roche Diagnostics, Germany). Paired serum samples were assayed for increasing titer against SARS CoV. Results: In the SARS epidemic, Artus and Roche PCR assays exhibited sensitivities of 87% and 85% for respiratory specimens (n = 64), 91% and 88% for stool (n=44), and 82% for urine (n = 29). A specificity of 100% was exhibited by both PCR assays except Artus attained only a 92% specificity for stool. For post-epidemic period, no SARS CoV was identified among 56 respiratory specimens by all PCR assays. Inhibitors to PCR assays were detected at an average rate of 7-8% among 202 clinical specimens. Conclusion: This study highlights the high throughput and performance of automatic RNA extraction in coordination with standardized real-time PCR assays suitable for large-scale routine diagnosis in case of future SARS epidemic. As no SARS CoV was detected among specimens collected during post-epidemic period, the positive predictive value of real-time PCR assays for detection of SARS CoV during low epidemic requires further evaluation.</div>
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Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods

Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavims during outbreak and post-epidemic periods

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri